MAG 1 analytical and MAG 0 micro

HPLC and LC columns - MAG 1 analytical and MAG 0 micro

While in preparative chromatography the separation efficiency of a column depends primarily on the method used to distribute the mobile phase uniformly across the entire internal area of the column, in analytical chromatography the quality of the internal column surface is of decisive character.  The sorbent particles in contact with the internal column wall are by necessity packed differently than those elsewhere; in the latter instance regularity of packing of spherical particles is close to that characteristic of a crystal lattice. 

Metallic columns MAGic 1 with internal diameter of 4.6 mm are manufactured and supplied with column length 50 mm, 150 mm or 250 mm.  Columns are manufactured from  top- quality stainless steel tubing which surface is electropolished.On both ends the column has threaded end pieces attached to the column by means of conical, two-edge seals in order to minimise the column stress and the resulting deformation. Stainless steel screen (2 mm mesh size) is held by means of a PTFE seal in a recess made in the inside of the end piece.  A cap screwed on the end piece presses the column cap with a recess for a sealing ferrule and the connecting capillary against the screen. The cap has a threaded through hole for the connecting capillary (external diameter 1.6 mm); the system allows both the metric type (M5, Europe) and inch type (UNF 10/32, US) through-bolts to be used.                                                 

MAGic1 columns are supplied together with a certificate. The maximum working pressure is 30 MPa. MAGic1 columns are packed with all sorbents manufactured by Labio with particle diameter 5, 7 and 10 mm, upon request and after consultation also with sorbents made by other manufacturers and with other particle diameters.

Columns for analytical liquid chromatography can be also supplied together with pre-columns MAP of the same diameter as the column proper. Precolumns are 20 mm long and are attached directly to the column by means of a pre-column bushing. The pre-column is closed on both ends by a screen held in place by means of a sealing ring. MAP precolumns are supplied non-tested, packed with all silica sorbents manufactured by Labio with particle diameter 5, 7 and 10 mm, upon request and after consultation also with sorbents made by other manufacturers and with other particle diameters.  

For many years microchromatography has been a promising scion of high-performance analytical chromatography. Unfortunately, low level of standardisation and high demands imposed on operation of microchromatographs prevent their more extensive use.

We in Labio have decided to design an instrument on the borderline between microchromatography and traditional analytical chromatography. Its advantage consists in that its operation is essentially identical with that of classical analytical systems while the volumes of columns and samples is about eight times smaller. We have also developed the corresponding microcolumns of type MAGic0. For microcolumns the quality of internal surface is more important still than for analytical chromatographic columns; accordingly, MAGic0 columns are made from glass tubing with inner diameter 1.4 mm, jacketed at elevated temperatures with stainless steel. End pieces are attached to the tubes with precision-ground ends by means of barrel-shaped ferrules made of the PEEK polymer. As in MAG 1 columns a stainless steel screen is used to contain the sorbent. In this instance the connecting capillary, held in place by a through-bolt, directly abuts the screen. As a result, a standard capillary of external diameter of 1.6 mm (1/16 ") and internal diameter of 0.2 or even 0.1 mm can be used.

Also MAGic0 columns are supplied with a certificate. The maximum working pressure is 300 bar. The columns are marketed packed with all sorbents manufactured by Labio with particle diameter of 5 mm, upon request and after consultation also with sorbents made by other manufacturers and with other particle diameters.

Also the microcolumns can be used along with pre-columns of type MMAP; these have the same diameter as the microcolumn, particle layer length of 20 mm, and are attached directly to the microcolumn without any bushing. On both ends of the precolumn there is a screen held in place by a sealing ring. MMAP pre-columns are marketed non-tested, packed with all silica sorbents manufactured by Labio with particle diameter 5 mm, upon request and after consultation also with sorbents made by other manufacturers and with other particle diameters.